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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of 293 cells using the different Nucleofection™ Platforms.
Different 293 subclones derived from different sources may have divergent features and display an extremely high genetic instability. Due to this variety between different 293 clones optimal Nucleofection™ Conditions may vary for each clone. This is the reason why we developed different protocols for the 293 clone CRL-1573 from American Type Culture Collection and the 293 clone ACC305 from DSMZ. For Nucleofection™ of other 293 clones, the optimal Nucleofection™ Conditions can easily be determined using the Cell Line Optimization Kits or alternatively please contact our Scientific Support Team for further information.
Example for Nucleofection™ of 293 cells
293 cells were transfected by Nucleofection™ with a plasmid encoding the fluorescent protein eGFP. 24 hours post Nucleofection™ , cells were analyzed by light (left) and fluorescence microscopy (right).