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Optimized Protocols indicating the optimal Nucleofector™ Kit and Conditions for a broad selection of cell lines are available and can be downloaded from:
The Cell Line Optimization Nucleofector™ Kit is the ideal tool for the transfection of virtually any difficult-to-transfect cell line with the Nucleofector™ 2b/II Device. It enables you to conveniently determine the optimal Nucleofection™ Condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector™ Solutions, V and L, each of which should be tested in combination with seven different Nucleofector™ Programs. Fine-tuning for optimal results can then be performed together with our Scientific Support Team. Nucleofection™ Conditions optimized with our Cell Line Optimization Kits using pmaxGFP™ Vector are equally well suited for other nucleic acid substrates. Thus, siRNA oligonucleotides can be transfected using the same conditions as with plasmid DNA.
One out of hundreds of success stories for cell line optimization assisted by our Scientific Support Team:
Results with SST support: up to 80% efficiency, up to 60% viability*
*for further details visit our cell transfection database