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The Cell Line Optimization Nucleofector™ Kits are the ideal tool to conveniently and rapidly determine the optimal Nucleofection™ Condition of virtually any difficult-to transfect cell line within one experiment. With the unique capability of the different Nucleofector™ Platforms (4D-Nucleofector™, HT Nucleofector™ and 96-well Shuttle™ System) to address individual wells of a 16-well, 96-well or 384-well Nucleocuvette™ Plate with different programs, cell line optimizations are easily performed within one experiment. In each system our three Cell Line Nucleofector™ Solutions SE, SF and SG are tested with a pre-selected set of programs plus controls.