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For optimal Nucleofection™ Performance with mouse T cells it is highly recommended to use Mouse T Cell Nucleofector™ Medium for cell culture steps post Nucleofection™.
The medium is included in our Mouse T Cell Nucleofector™ Kit (for the Nucleofector II/2b Device), and offered a separate product when using the P3 Kit with the 4D-Nucleofector™ System or the 96-well Shuttle™ Device.