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L7™ PBMC Reprogramming Bundle
robust generation of iPSCs from peripheral blood monunuclear cells (PBMCs).
various adult cell types including peripheral blood mononuclear cells (PBMCs), fibroblasts or CD34+ hematopoietic progenitor cells can be
reprogrammed to a pluripotent state by introduction of defined transcription
factors. The resulting induced pluripotent stem cells (iPSCs) appear to
be phenotypically and functionally equivalent to human embryonic stem cells
(hESCs). The ability of hESCs and iPSCs to self-renew in culture and their
potential to differentiate into cell types from all three germ layers makes
them a powerful research tool.
iPSC generation and potential applications:
Moreover, iPSCs also have great potential as a renewable
source of material for producing therapeutic cells for transplantation in
regenerative medicine. Because they can be derived from any individual, iPSCs
may be useful for generating autologous therapeutic cells for transplantation.
Nucleofector™ Technology for iPSC Generation
For human cells, this cellular reprogramming
process was first described in 2007 by two independent research groups
(Yu J et al. (2007) Science 318 (5858): 1917–1920;
Takahashi K et al. (2007) Cell 131 (5): 861–872). These reprogramming factors were
initially transferred into the somatic cells by retroviral transduction,
thereby diminishing their applicability to the clinic. Lonza’s Nucleofector™
Technology has been demonstrated to be a convenient, efficient, and
cost-effective non-viral alternative for iPSC generation and is currently being used by
leading scientists around the world.
Benefits of the Nucleofector™
Technology for iPSC generation include:
Guidelines for iPSC Generation from Various Starting Cell Types
a) Human PBMCs
reprogramming of peripheral blood mononuclear cells (PBMCs), Lonza’s
Pluripotent Stem Cell Innovation Team has developed an optimized protocol using
the 4D-Nucleofector™ System and episomal vectors. The protocol is accompanied
by supporting products, i.e. the L7™
PBMC Reprogramming Bundle.
For reprogramming of human dermal fibroblasts, Lonza
does not have a specific reprogramming protocol yet, but there are various
references available that can provide you with some details.
CD34+ hematopoietic progenitors
For reprogramming of human CD34+ hematopoietic
progenitors, Lonza does not have a specific reprogramming protocol yet, but
there are various references available that can provide you with some details.
Human Bone Marrow CD34+ Cells
Human Cord Blood CD34+ Cells
Besides the three main cell types used as
starting material for iPSC
generation, the Nucleofector™ Technology has also been used to reprogram
various other cell types.
Human Bone Marrow Mononuclear Cells
Human Neural Progenitor Cells
Human Adipose-derived Stem Cells
iPSC generation does not have to be a mystery with Lonza’s comprehensive
solutions - From the most
commonly used primary cell types for
reprogramming; to the Nucleofector™ Technology for
efficient, non-viral delivery of reprogramming factors; to the L7™ Culture System for
feeder-free, xeno-free culture of human iPSCs, Lonza provides a wide range of
products to aid in your generation and culturing of iPSCs.