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Human skeletal muscle myoblasts stained for desmin (green) and DAPI (blue)
Clonetics™ Human Skeletal Muscle Cells (SkMC) and Myoblasts (HSMM) are isolated from normal donors. HSMMs are isolated from the upper arm or leg muscle tissue, and SkMCs are isolated from gestational tissue usually from the quadriceps or psoas tissue. Both are sold in second passage. HSMMs can be differentiated to form myotubes in culture.
Clonetics™ HSMMs are guaranteed through 10 population doublings and to differentiate to form multinucleated myotubes in culture. SkMCs are guaranteed through 15 population doublings and to stain positive for desmin. Both cell types test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
Recommended medium: HSMMs require SkGM™-2 BulletKit™ and SkMCs require SkGM™ BulletKit™
guarantees the performance of Clonetics™/Poietics™ cells only if appropriate
Clonetics™/Poietics™ media and reagents are used exclusively and the
recommended storage and use protocols are followed. Any modifications made to
the recommended cell systems including the use of alternative media, reagents
or protocols, will void cell and media performance guarantees. If you need
assistance in selecting the appropriate media, reagents, or protocol, please
contact Lonza Scientific Support.