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Renal proximal tubule epithelial cells stained for γ-GTP
Most Clonetics™ Primary Cells are also available as proliferating cells in either flasks or plates, or as pellets in RNALater®.
Clonetics™ Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type. Human Renal Proximal Tubule Cells (RPTEC) are isolated from the proximal tubule. Renal Epithelial Cells (HRE) are a mixture of epithelial cells from the cortex and glomerular. Renal Cortical Cells (HRCE) are a mixture of proximal tubule and distal tubule. Mesangial Cells (NHMC) are isolated from the renal glomerulus and modified SMC between capillaries.
Clonetics™ Diabetic Renal Proximal Tubule Epithelial Cells are isolated from human donors that have been diagnosed with diabetes type II disease. Further donor information, including medications, BMI, and disease duration may be obtained by contacting Lonza Scientific Support. Diseased cells are grown in the same optimized media system as Clonetics™ Normal Human Renal Cells.
All cell types are guaranteed through 15 population doublings. RPTEC are guaranteed to test positive for gamma-GTP. HRE are guaranteed to test positive for pan cytokeratin. HRCE are guaranteed to stain positive for cytokeratin. NHMC are guaranteed to test positive for fibronectin and negative for cytokeratin 19 and von Willebrand Factor VIII. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
Recommended medium: RPTEC, HRE, HCRE use REGM™ BulletKit™ , NHMC use MsGM™ BulletKit™
Cell Specifications: Clonetics™ Cells are guaranteed to perform as indicated when used with Clonetics™ Media and Reagents.