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TRAP stained osteoclasts in culture.
The maintenance and repair of bone tissue is primarily attributed to two cell types: osteoblasts which form new bone tissue and osteoclasts which re-absorb bone tissue. Defects in the bone mineralization/bone degradation cycle contribute to a number of bone related diseases such as osteoporosis. Osteoclasts are large, multinucleated cells that play an active role in bone resorption. The Poietics™ Osteoclast Precursor Cell system has been designed for use in high-throughput applications to conduct research on osteoporosis, bone resorption, and other bone-related diseases.
Monocytes from the marrow or the blood
serve as precursors of osteoclasts. Traditionally,
researchers drive populations of purified monocytes to become osteoclasts, however, in some cases, this can result in a differentiation efficiency of as low as 10%. In contrast, Lonza's
Osteoclast Precursor Cell System yields up to 50%
conversion of precursors to osteoclasts when
Cryopreserved Poietics™ Human Osteoclast Precursor Cells (OCPs) are guaranteed to contain ≥1 million viable cells per ampoule and test positive for osteoclast activity and stain for TRAP following differentiation. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended Medium: OCP Octeoclast Precursor BulletKit™ Medium (provides both negative control/experimental medium which can be used as a negative control [without the addition of additional supplements] or an experimental medium [with the addition of researcher provided differentiation supplements] and a positive control medium guaranteed to induce differentiation into mature, functionally active osteoclasts; Each bottle of the OCP BulletKit™ Medium contains enough differentiation medium to fully differentiate approximately 1.5 million osteoclast precursor cells [approximately 1.5 complete 96-well plates] into mature, functionally active osteoclasts).
The OCP Medium is offered as a BulletKit™ Medium (catalog no. PT-8001) which includes both the basal medium and the necessary supplements for both plating and differentiation of osteoclast precursors. The basal medium (OCP Osteoclast Precur. Basal Medium; catalog no. PT-8201) and the necessary supplements (OCP Osteoclast Precur. SingleQuot Kit; catalog no. PT-9501) can also be purchased individually.
Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.