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A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures
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Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.
Adult and neonatal keratinocytes are also available.
Recommended Subculturing Reagents: ReagentPackTM Subculture Reagents
In a side-by-side comparison with a major competitor’s formulation, cultures maintained in KGM-Gold™ Keratinocyte Growth Medium had a more homogeneous phenotype consisting of small refractile cells with minimal enlargement and vacuolization. The ability of KGM-Gold™ Medium to promote the health of NHEK-Normal Human Epidermal Keratinocyte cells positively influences keratinocyte lifespan.
Figure 4A: Growth
of Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium compared to Competitor
A’s adult keratinocytes in their medium.
Figure 4B: Morphology of Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium compared to Competitor A’s adult keratinocytes in their medium.
Competitor A's adult keratinocytes in their medium
Day 13, 15 TPD
Day 25, 17 TPD
Day 20, 19 TPD
Day 32, 19 TPD
The growth performance and longevity of Competitor A's adult keratinocytes grown in Competitor A's Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.
New! Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 192627) and KGM™-Gold BulletKit™ Growth Media (catalog no. 192060)