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KGM™ Keratinocyte Growth Medium has been optimized for the proliferation of keratinocytes in a serum-free environment. KGM™-CD Chemically Defined Keratinocyte Growth Medium was designed as a serum-free, non-animal origin, chemically defined cell culture medium to support the isolation, growth and proliferation of primary human keratinocytes in culture.
Did you know our customers have used KGMTM Media also to culture immortalized corneal epithelial cells? Check out Jolly, et. al. publication that provides some guidelines to do this.
KGM™-CD is the only medium of its kind on the market today and can be used for a variety of applications associated with skin research, wound healing and dermal disorders. The KBM™-CD Basal Medium, KGM™-CD SingleQuots™ Kit and the KGM™-CD BulletKit™ allow customers the same flexibility as other Clonetics™ Media Products.
KGM™-CD is also able to support growth and proliferation of both adult and neonatal primary human dermal keratinocytes as compared to our non-defined KGM™-2 Medium - determined by total population doublings (total PDLs). There was excellent lot-to-lot consistency for each media tested where the KGM™-CD Medium supported nearly 25 population doublings in our testing while still maintaining normal keratinocyte characteristics.
This KGM™-CD media system is a complete, optimized system for isolating and growing primary human keratinocytes. Note: Coating plates with fibronectin or other non-animal derived matrix material is necessary for successful keratinocyte isolations with KGM™-CD medium.
Note: Antibiotics are not included with the SingleQuots™ Kit (CC-4456) and must be purchased separately.
For more information on the discontinuation of certain keratinocytes products, click here