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Clonetics™ diseased fibroblasts include lung fibroblasts isolated from adult lung tissue of asthma, COPD and cystic fibrosis patients.
The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Important research applications includ pulmonary disease, i.e. COPD, asthma, CF, TB, cancer.
Cryopreserved Clonetics™ Diseased Lung Fibroblast cells stain negative for von Willebrand factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended medium: FGM™-2 BulletKit™
guarantees the performance of Clonetics™/Poietics™ cells only if appropriate
Clonetics™/Poietics™ media and reagents are used exclusively and the
recommended storage and use protocols are followed. Any modifications made to
the recommended cell systems including the use of alternative media, reagents
or protocols, will void cell and media performance guarantees. If you need
assistance in selecting the appropriate media, reagents, or protocol, please
contact Lonza Scientific Support.
Note: Learn more about our additional products for airway research.
Note: Contact Scientific Support for detailed donor information including age of diagnosis, medications taken, and smoking history.