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Cord blood mononuclear cells (MNC) are blood
cells containing a rounded nucleus (including macrophages, monocytes, and
lymphocytes). Cord blood hematopoietic stem cells (HSC) and hematopoietic progenitor cells (HPC) can be readily isolated from the mononuclear cell (MNC) fraction.
Cyropreserved Poetics™ Cord Blood Mononuclear Cells (MNC) are isolated from
whole cord blood via density gradient centrifugation and are guaranteed to contain either ≥50 million (2C-150B), ≥100 million (2C-150A), or ≥200
million (2C-150) viable cells per ampoule.
All donors and/or cell lots test
negative for HIV-1, hepatitis B and hepatitis C. A Certificate of Analysis is
provided for each cell lot purchased.
Medium: HPGM™ Hematopoietic Growth Medium for serum-free culturing
(additional cytokines may be necessary depending on application). Alternative media containing
serum may also be acceptable. Please contact Scientific Support for serum
containing media recommendations.
Lonza guarantees the performance of
Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and
reagents are used exclusively and the recommended storage and use protocols are
followed. Any modifications made to the recommended cell systems including the
use of alternative media, reagents or protocols, will void cell and media
performance guarantees. If you need assistance in selecting the appropriate media,
reagents, or protocol, please contact Lonza Scientific Support.