Bone marrow is the soft tissue found in the interior of bones and is
the origin of hematopoiesis, the process in which blood cells are derived from
hematopoietic stem cells (HSC). Stem cells require specific microenvironments,
or niches, for regulation of proliferation and differentiation.
The bone marrow niche contains various types of bone marrow cells, including hematopoietic stem
and progenitor cells (HSPC), osteoblasts, mesenchymal stem cells (MSC), Chimeric Antigen
Receptor (CAR)-expressing cells, and stromal cells1.
There are two known subsets of the bone marrow niche - the osteoblast niche and the vascular
niche. While there is still debate as to the exact mechanisms, it appears as
though the osteoblast niche is responsible for self-renewal and maintenance of long-term
HSCs (LT-HSCs), while the vascular niche is responsible for the mobilization of
HSCs into the bloodstream where they differentiate into mature blood cells2.
Bone marrow cells are of increasing interest not only for their ability
to reconstitute hematopoiesis, but also for a more thorough understanding of
bone marrow niches and bone marrow cells’ specific roles in hematopoiesis, the
immune system, and human disease.
When comparing bone marrow cells to other sources of hematopoietic cells,
bone marrow cells are unique in that they provide researchers the ability to work
with large numbers of cells from a single donor or investigate differences in donors
of various ages, genders, or ethnicities.
Lonza understands the importance of being able to compare and contrast
characteristics and functions of cells from various donors, which is why most
cell types are available from a variety of donors. Lonza Scientific Support
Specialists are readily available to answer questions you have about obtainable
Lonza Poietics™ purified bone marrow cells include:
Poietics™ bone marrow cells are guaranteed to have at least the viable cell
number claimed on the vial and purity levels are verified via flow cytometric
analysis. Mesenchymal stem cells, which are known to differentiate into chondrogenic, osteogenic, adipogenic and neural lineages, are also available.
1. Bone marrow vascular niche: Home for
hematopoietic stem cells. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009113/
2. Targeting the molecular and cellular
interactions of the bone marrow niche in immunologic disease. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932436/
Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.