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Mouse brain cortex neurons
Ready-to-use Clonetics™ Mouse Brain Cortex Neurons are isolated from Mouse C57 black brain (E14, 15) or Mouse CD1 brain (E14,15). These are sold as cell suspensions of high quality primary embryonic brain neuronal cells (including glia) prepared by standardized methods, and are ready for immediate culture.
Each vial of cortex cells contains approximately 4 million viable cells.
This will seed into 10 or more wells of a 24-well plate and 50 or more wells of a 96-well plate using the recommended plating densities and medium. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal.
Recommended medium: PNGM™ BulletKit™