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What Is a Primary Cell Culture?
Primary Cells – Cells isolated directly from human or animal tissue using enzymatic or mechanical methods. Once isolated, they are placed in an artificial environment in plastic or glass containers supported with specialized medium containing essential nutrients and growth factors to support proliferation. Primary cells could be of two types – adherent or suspension. Adherent cells require attachment for growth and are said to be anchorage dependent cells. The adherent cells are usually derived from tissues of organs. Suspension cells do not require attachment for growth and are said to be anchorage independent cells. All suspension cells are isolated from blood system. With the exception of cells derived from tumors, most primary cells have limited lifespan. These early passage cells may also contain heterogeneous cell populations.
Why Use Primary Cells:
Primary Cells vs. Cell Lines
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Learn how primary cells are different from cell lines
Your Tools to Get Started on Primary Cell Culture:
Lonza's Bulletkit Medium
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