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Human skeletal muscle myoblasts stained for desmin (green) and DAPI (blue)
Most Clonetics™ Primary Cells are also available as proliferating cells in either flasks or plates, or as pellets in RNALater®.
Clonetics™ Human Skeletal Muscle Cells (SkMC) and Myoblasts (HSMM) are isolated from normal donors. SkMCs are isolated from the upper arm or leg muscle tissue, and HSMMs are isolated from post-gestational tissue usually from the quadriceps or psoas tissue. Both are sold in second passage. HSMMs can be differentiated to form myotubes in culture.
Clonetics™ HSMMs are guaranteed through 10 population doublings and to differentiate to form multinucleated myotubes in culture which stain positive for desmin. SkMCs are guaranteed through 15 population doublings and to stain positive for desmin post differentiation. Both cell types test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provied for each cell lot purchased.
Recommended medium: HSMMs require SkGM™-2 BulletKit™ and SkMCs require SkGM™ BulletKit™
Cell Specifications: Clonetics™ Cells are guaranteed to perform as indicated when used with Clonetics™ Media and Reagents.