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In the image above, samples of E. Coli total RNA were denatured using the following denaturants: Lane A: Formaldehyde/ Formamide; Lane B: Formamide; Lane C: Glyoxal. Samples were loaded at 2 µg/lane for the formaldehyde/formamide and formamide only denatured samples, and 4 µg/lane for the glyoxal denatured samples. Reliant® RNA Precast Agarose Gels were run at 7 V/cm for 40 minutes in 1X MOPS Buffer and post stained with SYBR® Green II Gel Stain and photographed on the Clare Chemical Research, Inc., Dark Reader® Transilluminator.
For ssDNA and RNA, SYBR® Green II offers all the advantages of SYBR® Green I and is ideal for non-isotopic detection of DNA mutations by single-stranded conformation polymorphism (SSCP) analysis.