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Speed-Analysis of Undirected Migration of NK-92 Cells under the
Influence of the Adipokine Leptin
Our workgroup at Prof Kielstein’s lab investigates the link between
obesity, the adipokine leptin and the influence of leptin on natural killer
(NK) cells. Obese individuals have a higher likelihood to get various types
of cancer (e.g. postmenopausal breast cancer, hepatic- and colon cancer)1 and do
have a higher recurrence- and death probability compared to lean patients2.
One of the possible reasons has been demonstrated by our workgroup – we
could show that the cytotoxicity of NK cells is impaired under the influence of
elevated leptin concentrations in obese individuals3.
It has also been shown that leptin affects the remodeling of the
structural protein actin4. As actin is one of the main proteins
involved in active cell migration, we hypothesized that higher concentrations
of leptin might affect the migration speed of NK cells as well.
We used the CytoSMART™ System as an affordable and
easy-to-use system to create time lapse recordings of the undirected migration
of NK cells under the influence of physiological and pathophysiological (equal
to normal weight and obese individuals) concentrations of leptin.
The recorded videos have been analyzed by the TrackingTool™ PRO cell tracking software (Gradientech, Sweden) regarding the average
migration speed of the cells.
System completely fulfills our needs and is an adequate option for all
cell-tracking tasks where a high resolution or fluorescence is not required.
The big advantage in comparison to “traditional” live cell imaging systems
certainly is the small size and the possibility to use the device in your
already existing incubator, with no need for further investments.
Find out more about Prof. Kielstein’s research.
Interested to present your research here? Then apply
to become a CytoSMART™ Lab. You will receive a CytoSMART™ System on loan for 4
weeks and your project will be featured on the Lonza website.
How to become a CytoSMART™ Lab