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Insect-XPRESS™ Medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). Cell densities in excess of 8.3 × 106 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono¬layer cultures and shake-flask cultures. Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS). For cryopreservation of insect cells, Insect-XPRESS™ can be mixed 50:50 with Cryoprotective Medium (Cat. No. 12-132).