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Pro293™ Chemically Defined, Serum-free Media were optimized to support high-density growth and recombinant protein production in 293 neonatal kidney cells. They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal-origin components.
— Pro293™s Medium — for 293 cells growing in suspension culture
— Pro293™ a Medium — for 293 cells growing in adherent culture
Both media do not contain L-glutamine or phenol red. Pluronic® F-68 is present at 0.1%.