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UltraMEM™ Reduced Serum Media are chemically defined media designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations (see table). When supplemented with 2 – 4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages. In addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all. UltraMEM™ Reduced Serum Media are offered as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/ml.
– Growth and maintenance of anchorage-dependent cell types