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UltraCULTURE™ Serum-free Medium is a complete, allpurpose medium designed for the cultivation of a wide variety of adherent and non-adherent mammalian cell types. UltraCULTURE™ Medium can be used to support fusion of cells during hybridoma formation, growth of monocyte, macrophage, epithelial, and fibroblastic cell lines, and generation of virus particles for vaccine production. The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-132A) to cryopreserve cells in a serum-free environment. UltraCULTURE™ Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior to use.
The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to crossreference the Master File may be obtained by contacting the Regulatory Affairs Department.
– Cultivation of adherent and non-adherent mammalian cells
– Generation of viral particles for vaccine production
Performance and Quality Tests
– Turbidity may develop in UltraCULTURE™ Medium; experiments have determined that the turbidity will not alter the performance of the product