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Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension. It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8. After treatment, trypsin activity can be halted by adding a serum media mixture or a Trypsin inhibitor (from soybean, for an example) in serum-free systems.
Trypsin-EDTA is a component of ReagentPack™ Subculture reagents.