Introduction
Unparalleled Versatility
Based on 25 years of innovation in microbial biotechnology, Lonza now offers the XS Microbial Expression Technologies™ Platform, as an advanced and versatile biopharmaceutical production service.
No single strain or vector is capable of being the best expression option for all types of biopharmaceuticals. Therefore, Lonza has assembled a collection of proprietary expression systems that are screened in a high throughput manner to identify the best production clone for your product. Our XS Technologies™ encompass a diverse assembly of expression systems that not only deliver high expression levels but also quickly enable the creation of a productive and efficient cGMP processes.
To learn more about our diverse XS Technology™ from an industry expert, Dr. Joachim Klein, please download our recent webinar titled "Using the XS Toolbox to Optimize Strain Design and Development."
For more information on our microbial expression technologies, please download our XS Technologies™ Brochure.
Sugar Inducible E. coli
One of the most popular XS Technologies™ is Lonza’s sugar-inducible E. coli system.
This proven technology is comprised of a positively and tightly
regulated expression system that is induced by either rhamnose or
melibiose. Lonza's patented sugar-inducible systems have successfully
been used in over 12 projects and are capable of achieving soluble
expression levels well over 20 g/L. While the technology has shown it
can express many types of proteins, it has established a track record
for producing high-quality, correctly folded antibody fragments (including Fabs and scFv). Our highly trained development team can put together a package for custom Microbial Process R&D Services and early clinical production tailored to your pipeline needs.
System Includes:
- 4+ different E. coli strains
- 4+ plasmid backbones with stabilizing elements
- 2+ promoters
- Numerous secretion signal options
- Maintained without antibiotic resistance markers options for either monocistronic or polycistronic expression
- Plasmid variants with different folding catalysts assist in folding complex proteins such as antibody fragments
- The same mulitcloning site for all plasmids
- Options for intracellular, periplasmic or extracellular secretion
Benefits:
- High soluble expression
- Yields high-quality, correctly folded protein
- Product easily recovered from the periplasm or released extracellularly
- Extremely low background
- Low-cost, non-toxic inducer
Alternative Microbial Hosts
Lonza's has recently expanded its XS Technology™ Toolbox with the addition of two new alternative host organisms that address the wide diversity of microbially-derived products. The new hosts include, Pichia pastoris and Bacillius subtilis as well as expanded strain development and production systems. This newly expanded XS Toolbox™ provides complementary expression systems that quickly deliver simple, reliable, high yielding cGMP processes.
Pichia pastoris
Pichia pastoris presents an expression option for complex proteins as an alternative to the more commonly used E.coli expression. As a host organism, P. pastoris offers the benefits of both protein secretion and lack of endotoxin production, allowing for simplified recovery and downstream processing. In addition, our XS™ Pichia system also includes two different hosts that have been selected for their excellent growth properties and novel promoters. These characteristics in conjunction with a novel signal sequence will allow you to achieve high cell densities, high titers, and improved product quality. Our process development experts have also developed several Pichia expression processes that are methanol free, removing the complication of a solvent based process.
Bacillus subtilis
Lonza’s XS™ Bacillus subtilis system is an ideal option for the expression of monomeric protein products at expression levels up to 20g/L. This high yield, combined with our patented fermentation protocols allow for fast process development and scale-up to support early clinical testing.
