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For almost a decade the Amaxa™ Nucleofector™ Technology has proven to be the method of choice for non-viral transfection of hard-to-transfect cell types. A unique combination of electrical parameters, cell-type specific Nucleofector™ Kits and Optimized Protocols helps to achieve immediate transfection success in virtually any cell type.
Transfection efficiencies achieved with the Nucleofector™ Technology for primary neural cells. Different primary neural and glial cells were transfected by Nucleofection™ with a plasmid encoding either eGFP or eYFP (cortical rat neurons, data courtesy of J. Köhler, R. Klein, MPI for Neurobiology, Munich, Germany). Transfection efficiencies were determined by fluorescence microscopy or flow cytometry after 24 - 72 hours.
Nucleofector™ Kits for Primary Neural Cells
Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of primary neurons using different Nucleofection™ Platforms: