Mouse cortical neurons - Nucleofection™ in adherence (Y unit) after 6DIV

What about using a non-viral transfection method that achieves up to 70% transfection efficiency in primary neural cells?

For almost a decade the Amaxa™ Nucleofector™ Technology has proven to be the method of choice for non-viral transfection of hard-to-transfect cell types. A unique combination of electrical parameters, cell-type specific Nucleofector™ Kits and Optimized Protocols helps to achieve immediate transfection success in virtually any cell type.

 

  • Non-viral transfection of primary neural cells with up to 70% efficiency
  • Preservation of functionality
  • Different device platforms for different cell numbers and throughput options
  • NEW: 4D-Nucleofector™ System allows transfection of 2x104 to 2x107 cells with same protocol and with up to 16 reactions in parallel
  • NEW: 4D-Nucleofector™ System enabling adherent Nucleofection™ of primary neurons or glial cells after several days of culture


Transfection Efficiencies of Primary Neural Cells post Nucleofection™
Transfection efficiencies achieved with the Nucleofector™ Technology for primary neural cells. Different primary neural and glial cells were transfected by Nucleofection™ with a plasmid encoding either eGFP or eYFP (cortical rat neurons, data courtesy of J. Köhler, R. Klein, MPI for Neurobiology, Munich, Germany). Transfection efficiencies were determined by fluorescence microscopy or flow cytometry after 24 - 72 hours.



Nucleofector™ Kits for Primary Neural Cells

Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of primary neurons using different Nucleofection™ Platforms:



Nucleofection™ Platforms: