Stefanie Buesch1, Sabine Schaepermeier1, John Langer2, Claudia Schwartz1, Jenny Schroeder1

1Lonza Cologne GmbH, Cologne, Germany; 2Lonza Walkersville Inc., Walkersville, MD, USA

 

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 Video of tube formation assay with HUVEC cells recorded with CytoSMART™ System  

Supplementary material for figure 2: Video of Tube Formation Assay

Human Umbilical Vein Endothelial Cells seeded on BME and the formation of endothelial tubes was monitored live with the CytoSMART™ Lux 10X System. Time-lapse videos were recorded for 22 hours at an image interval of 1 image every 5 min with the CytoSMART™ Lux 10X System.

     
 Video of scratch assay with HCT-116 cells recorded with CytoSMART™ System  

Supplementary material for figure 5: Video of 2D Cell Migration Assay

The human colorectal carcinoma cell line HCT 116 was seeded into 35mm micro-dishes containing an ibidi culture insert. After reaching confluence and removal of the culture insert, closure of the gap was monitored with the CytoSMART™ Lux 10X System at an image interval of 1 image every 5 min for 1.8 days.

     
 Video of invasion assay with U-87 cells recorded with CytoSMART™ System  

Supplementary material for figure 7: Video of 3D Invasion Assay

Sprouting of cell line U-87 MG from a spheroid structure into a 3D matrix was recorded for 2.8 days at an image interval of 1 image every 60 min with the CytoSMART™ Lux 10X System.

  

Poster information:

Poster title: Simple and Easy Monitoring of Tube Formation and Migration Assays with the CytoSMART™ Live Cell Imaging System

Session Date and Time: Sunday 2 April 2017, 1:00 PM - 5:00 PM

Session Title: Cell Culture and Animal Models of Cancer 1

Location: Poster Section 38

Poster Board Number: 28

Session Category: Tumor Biology

 

 

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