Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages associated with the use of serum-free media formulations. These include:

- Increased definition

- Increase lot-to-lot consistency

- Simplify purification and processing

- Improved control of cell culture conditions

- Optimize formulations for specific cell types

 


PowerCHO™ 1, 2, and 3    Chemically Defined, Serum-free CHO Media are the next generation in CHO media, optimized for both cell growth and protein production. PowerCHO™ Media are hydrolysate-free, serum-free, and non-animal origin media for supporting high-density CHO cells in suspension. For therapeutic bioprocessing applications, these protein-free formulations also facilitate both downstream purification and regulatory compliance.

PowerCHO™ contains HEPES and Pluronic® F-68, but does not contain L-glutamine, phenol red, hypoxanthine, and thymidine.

 

ProCHO™ 4, 5 and AT Protein-free CHO Media   were developed specifically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These proteinfree formulations support high-density cultures without the need for animal derived components. Very low levels of recombinant insulin facilitate both downstream purification and regulatory compliance. The following media systems are available:

 - ProCHO™ 4 Medium — For concurrent transition of adherent CHO cells to serum-free and suspension culture; supports faster doubling times

- ProCHO™ 5 Medium — For CHO cells already growing in suspension; supports increased protein production

- ProCHO™ AT Medium — For adherent culture of CHO cells

 

UltraCHO™ Serum-free CHO Cell Media   are optimized to support the growth of transfected and non-transfected Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins. UltraCHO™ Media are composed of a modified DMEM:F-12 base. The formulation is supplemented with insulin, transferrin, and proprietary purified proteins. Media contains less than 300 μg/ml protein. UltraCHO™ Media are manufactured under FDA current GMPs and the formulation has been submitted to the FDA as a Master File.

 

XTREME™ Feed is a non-animal orgin, protein-free and chemicially defined feed to be used with PowerCHO™ 2, and ProCHO™ 4, and -5. The low salt content allows for a good control and finetuning of the medium osmolality.

 

PowerFeed™ A    and  PowerFeed A with Lipidscan be used with all PowerCHO and ProCHO media. They are non-animal origin, protein-free and chemically defined. Powerfeed A with lipids contains a mix of cholesterol and fatty acids to support the growth of cells which need lipids.

 

ProFreeze™-CDM , NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS). However, it is used to greatest advantage with cells cultured in a serum-free and animal component-free environment. This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.