car-t immune cells

Lonza provides the CAR-T market with end-to-end solutions for successful commercialization. This includes tools that can be used to develop and test therapeutics, ranging from cell culture and discovery technologies to quality control tests and process optimization. One of the latest additions to the portfolio is the 4D-Nucleofector™ LV-Unit . Designed to provide a closed system in which to transfect cells and successfully generate various cell types that can be used in the CAR-T process and beyond.

Thank you for visiting our Lonza Booth at the 2016 CAR-TCR Summit. Explore how we can support your CAR-T research.

LV Unit  

4D-Nucleofector™ LV Unit


The LV Unit, a new functional unit for the 4D Nucleofector™ System, allows for closed, scalable transfection of larger cell numbers in the range of 1x107 to 1x109 cells. Transfection protocols can be established in smaller scale using the X Unit and subsequently transferred to the LV Unit without the need for re-optimization.


  • Ex-vivo modification of human primary cells for cell therapy applications (e.g. genome editing, generation of CAR-T cells)
  • Transient production of potential therapeutic proteins or antibodies for construct screening
  • Generation of large numbers of transiently modified primary cells for cell-based assays

x-vivo bottle

X-Vivo™ Medium 


Hematopoietic Cell Medium that provides nutritionally complete and balanced environments for a variety of cells.  X-Vivo™ Medium has also been shown to work well with T-cells in a variety of CAR-T applications with the addition of specific supplements.


X-VIVO™ Medium is available in three formulations:      

  • X-VIVO™ 15 Medium: Richest medium to support most cell types: human and mouse CD34+ cells, CD4+ T cells, leucocytes, neutrophils, dendritic cells, human bone marrow MNC’s.
  • X-VIVO™ 10 Medium: Human ES cells, Lymphocytes
  • X-VIVO™ 20 Medium: Peripheral Blood Mononuclear Cells (PBMC’s)
  • X-VIVO™  10 and 15 Media available in non-animal origin formats

 Coming soon: New T-cell specific X-VIVO™ Medium

man in the lab

Lonza’s CMO Services


Lonza Custom Manufacturing services extend across the entire value chain of the CAR-T sector. Raw material supply of high quality GMP viral particles for successful on-demand immune cell transduction includes development of your virus seed through process validation. With 20 years of viral GMP manufacturing experience (AAV, adeno, lenti, and others), we can supply product to cover our clients’ needs from preclinical to commercial application.  Our process development scientists work hand-in-hand with our clients to develop client-specific custom protocols using a variety of manufacturing platforms.  We meet each unique need, encompassing every aspect of the business from raw material requirements, process flow, scalability, closed systems, automated vialing, to storage and distribution.